Measurement
pXS-Oxy4

Part:BBa_K2512008:Design

Designed by: Huang Nan   Group: iGEM17_Worldshaper-XSHS   (2017-10-22)


vgb promoter+T7RNA polymerase+T7 promoter+GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3515


Design Notes

The part BBa_K2512007 contains a T7 promoter and a GFP coding sequence. The T7 promoter was synthesized by Genscript, which was used as a primer to amplify the GFP sequence in part BBa_E0840.


Source

BBa_K561001 and BBa_K145001 were obtained from 2017 Distribution Kits. The part BBa_K2512007 was constructed by our group.

References